• In vivo nocieptive synaptic transmission and its plastic change

The spinal dorsal horn, especially superficial dorsal horn, has an important role in the transmission and modulation of nociceptive information. Superficial dorsal horn neurons make excitatory synaptic contacts with noxious primary afferent fibers. In addition, dorsal horn neurons receive inhibitory synaptic inputs from spinal interneurons and descending inhibitory neurons in the brain stem. We investigate the spinal modulatory mechanism of nociceptive transmission by using in vivo patch-clamp recording techniques (Figure). We also study the underlying mechanism for the development of chronic pain. Behavioral and immunohistochemical analyses are also used.

Figure. In vivo patch-clamp recording from spinal dorsal horn neuron.

1. Furue H*, Katano T*, Okuda-Ashitaka E, Tagaya M, Watanabe M, Yoshimura M and Ito S (*contributed equally). N-Ethylmaleimide-sensitive fusion protein (NSF) is involved in central sensitization in the spinal sord through GluR2 subunit composition switch after inflammation. European Journal of Neuroscience 27(12):3161-3170, 2008

2. Furue H* , Suzuki SC*, Koga K, Jiang N, Nohmi, M, Shimazaki Y, Katoh-Fukui Y, Yokoyama M, Yoshimura M, Takeichi M (*contributed equally). Cadherin-8 is Required for the First Relay Synapses to Receive Functional Inputs from Primary Sensory Afferents for Cold Sensation. Journal of Neuroscience 24:3466-3476, 2007

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