洪洋洋（Hong Yangyang）さん

During the first two-week rotation in the Genetics Department, I had the privilege of meeting Professor Ohmuraya. He presented the essence of genetics to us in a clear and vivid manner. What made the experience special was his kind and sincere communication—whether discussing experimental work or having a relaxed conversation, his enthusiasm made every interaction extremely pleasant. Under Dr. Imasaka's guidance, we performed Sanger sequencing, learning how to prepare DNA samples, set up sequencing reactions, and analyze the results. I also helped validate conditional knockout mouse genes through genotyping PCR and gel electrophoresis. Some experiments had unsatisfactory results due to contamination or technical issues, but others worked well, teaching me the importance of problem-solving and careful technique. Additionally, under the guidance of senior researchers Wenting and Jingren, we observed the mouse sacrifice procedure and liver sample collection. With Dr. Okuno and senior researcher Jingren, I observed and practiced complete Western blot and PCR workflows, including protein extraction, gel preparation, sample loading, gel running, membrane transfer, antibody staining, blocking and detection. These experiences improved my pipetting accuracy, attention to detail, and understanding of molecular biology principles. The department's collaborative atmosphere—where senior researchers patiently guided me and peers shared tips—made learning enjoyable. This rotation not only strengthened my technical skills but also showed me the value of teamwork and persistence in research.

In the third week, during the rotation in Lower GI Department, I encountered the robot-assisted surgery. For the first time, I observed how surgical robots function in actual operations. Observing procedures performed with the da Vinci and Japan’s proprietary Hinotori surgical robots was impressive. During the operation, we talked about the advantages of Robot-assisted surgery with Dr. Kimura. Dr. Kimura highlighted their advantages, including 3D visualization, multi-articulated arms, enhanced precision with stable robotic arms and instant tool transitions (e.g., electrocautery → scissors). Before the operation, Professor Ikeda would point to the imaging report and explain to us where the lesion was located in this case and the extent of the surgical removal. During surgeries, Dr. Kimura and senior resident Zhenxin explained anatomical landmarks and procedural steps in detail, such as pointed out the optimal location for peritoneal incision and where we can find the inferior mesenteric artery and how to isolate it. After the resection of the colorectal cancer specimens, we examined it with our own hands, palpating their textural differences from healthy tissue—an invaluable tactile learning opportunity. This direct contact is more likely to leave a deeper impression on our minds than simply memorizing the conclusions in the pathology textbooks. In addition, during outpatient clinics with Professor Ikeda, I observed his exemplary patient-centered approach. Professor Ikeda treated each patient very gently and did not seem to be in a hurry at all. Before each consultation, he would introduce the case to us briefly about the patient’s diagnosis, imaging findings, and treatment plan. During the everyday ward round, notably, the use of privacy curtains around beds demonstrates respect for the patients' privacy. What surprised me even more was that each bed was surrounded by curtains. Not just the special beds had curtains, but every single bed did.

Finally, my rotation was in the Department of Respiratory Medicine. In this unit, Dr. Takahashi guided us through ICU and general ward rounds, for patients with SARS-CoV-2 and COVID-19, they have been isolated in specialized wards and are not accessible. The next day, we followed Professor Kijima to the outpatient clinic. Before each patient entered, Professor Kijima would introduce the diagnosis of the patient, as well as the relevant treatment and histological classification of lung cancer. And the senior resident would explain it in detail to us. This enables us to better understand the cases in the outpatient department. In the following days, we observed the operation of bronchoscopy. The doctors explained the cases of each patient and the necessary procedures, such as bronchoalveolar lavage and biopsy tissue collection. On the last day, we also conducted a simulation practice of bronchoscopy, where they taught us how to operate the handle to move the lens up and down, as well as the specific positions and names of each lung segment during the visual operation of the bronchoscope. We gained a lot from the hands-on practice.

In conclusion, my one-month summer experience at Hyogo Medical University has been an invaluable experience that deepened my practical skills in molecular biology and clinical observation, while also broadening my understanding of Japanese medical culture. Through hands-on work in genetics research (Sanger sequencing, Western blot, PCR) and clinical shadowing in robotic surgery and respiratory medicine, I gained firsthand insight into both laboratory techniques and patient-centered care. I sincerely thank Professor Ohmuraya, Professor Ikeda, Professor Kijima and all mentors and peers who made this experience so rewarding. This opportunity has not only strengthened my technical and clinical competencies but also reinforced my passion for bridging research with practical medicine.